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Millipore Anti-Dimethyl-Histone H3 (Lys9) Antibody, Alexafluor® 488 Conjugate Is An Antibody Against Dimethyl-Histone H3 (Lys9) Antibody For Use In Immunocytochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
Product Information
Format
AlexaFluor®488
Presentation
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
Quality Level
MQ100
Applications
Application
Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor® 488 Conjugate is an antibody against dimethyl-Histone H3 (Lys9) Antibody for use in Immunocytochemistry.
Key Applications
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl-Histone H3 (Lys9) in HepG2 cells. The unconjugated antibody (Cat. No. 07-441) is shown to be suitable also for Western blotting, dot blot, immunocytochemistry, and peptide inhibition assay (peptide blocking) applications.
Biological Information
Immunogen
KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..RKme2STG.., in which Kme2 corresponds to dimethyl-lysine at residue 9 of human Histone H3.
Epitope
H3K9me2
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Histone H3 dimethylated on lysine 9.
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse. Predicted to react with Yeast, Rat, Chicken based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_003493
Gene Symbol
HIST3H3
H3FT
Purification Method
Protein A Purfied
UniProt Number
Q16695
Molecular Weight
~17 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected dimethyl-Histone H3 (Lys9) in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
