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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma | ELISA, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5612 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Citrulline Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit antiserum. Liquid with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC. |
| Key Applications |
|
| Applications Not Recommended |
|
| Application Notes | Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues. ELISA: 1:2500-1:5000 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Citrulline-Glutaraldehyde-BSA |
| Host | Rabbit |
| Specificity | Citrulline in glutaraldehyde fixed tissues or cells. The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds: Compound Cross-reactivity ratio Citrulline-G-BSA 1 Arginine-G-BSA 1/>100,000 Glutamine-G-BSA 1/>100,000 Ornithine-G-BSA 1/>100,000 Abbreviations: (G) Glutaraldehyde (BSA) Bovine Serum Albumin |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |