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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5675 |
| Replaces | AB1596; 06-451 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Metabotropic Glutamate Receptor 5 Antibody, pain |
| Alternate Names |
|
| Background Information | Metabotropic glutamate receptor 5 or mGluR5 (UniProt Q3UVX5; also known as G protein-coupled receptor GRM5, G protein coupled receptor family C, group 1, member E) is encoded by the Grm5 (also known as Gprc1e, Mglur5, AI850523, 6430542K11Rik) gene in murine species (Gene ID 108071). The metabotropic glutamate receptors belong to a family of G protein-coupled receptors that have been divided into 3 groups based on their sequence homology, putative signal transduction mechanisms, and pharmacologic properties. mGluR1 and mGluR5 constitute Group I metabotropic glutamate receptors. mGluR5 is found on postsynaptic terminals of neurons and microglia. mGluR5 signals via Gq/G11 coupling to activate phospholipase C, resulting in calcium mobilization and activation of protein kinase C (PKC). In addition, mGluR5 is also reported to mediate signaling involving Src-family protein tyrosine kinase in a G protein-independent manner. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Metabotropic Glutamate Receptor 5 Antibody, pain is an antibody against Metabotropic Glutamate Receptor 5 for use in IH & WB. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.1-0.5 μg/mL using ECL on rat brain microsomal preparation. Reacts with a band of ~132 kDa. Immunohistochemistry: 0.1-0.2 μg/mL on rat brain and mouse brain sections fixed with 4% paraformaldehyde containing 15% saturated picric acid in 0.1M PB, pH 7.4. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Metabotropic Glutamate Receptor 5 in mouse hindbrain and rat colon tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the cytoplasmic domain of mouse Metabotropic Glutamate Receptor 5. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody will not detect Isoform 2. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Rat. Predicted to react with Human, Bovine, Primate, Feline, Horse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternative splice variants of GRM8 have been described but their full-length nature has not been determined. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P41594 # Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system and generates a calcium-activated chloride current. SIZE: 1212 amino acids; 132469 Da SUBUNIT: The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP (By similarity). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P41594 ## Belongs to the G-protein coupled receptor 3 family. |
| Molecular Weight | ~150 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in microsomal preparation of rat brain tissue lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Metabotropic Glutamate Receptor 5 in 10 µg of microsomal preparation of rat brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |