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Key Specifications Table
| Key Applications |
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| CULT |
| Description | |
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| Catalogue Number | CC076 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Human Collagen Type IV |
| Overview | Collagen type IV is the major structural element of basal membranes. Human collagen type IV is a native triple helix. Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type IV was purified by using differential salt precipitation. Purity and retention of native helical structure was controlled by SDS-PAGE and by reaction with anti-collagen type specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL |
| Product Information | |
|---|---|
| Presentation | Liquid purified protein. In 100 μL of 0.5M acetic acid solution, pH 2.5. Salt-free, no preservative. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Application Notes | Coating material for cell culture studies Formation of collagen gels Optimal working dilutions must be determined by end user. |
| Biological Information | |
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| Concentration | 1 mg/mL |
| Purity | Human collagen type IV: 95% pure by SDS-PAGE. Human collagen types I-III, V, VI, and non-collagen proteins:<5%. Retention of native structure was confirmed by ability to form microfibrils. |
| Source | Human placenta, negative for HBsAg and HIV antibodies |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P02462 # Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Potently inhibits endothelial cell proliferation and angiogenesis. Inhibits angiogenesis potentially via mechanisms involving cell surface proteoglycans and the alpha and beta integrins of endothelial cells. SIZE: 1669 amino acids; 160615 Da SUBUNIT: There are six type IV collagen isoforms, alpha 1(IV)- alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. TISSUE SPECIFICITY: Highly expressed in placenta. DOMAIN: SwissProt: P02462 Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G- X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain. PTM: Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates. & Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. & The trimeric structure of the NC1 domains may be stabilized by covalent bonds between Lys and Met residues. DISEASE: SwissProt: P02462 # Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant. & Defects in COL4A1 are a cause of porencephaly type 1 [MIM:175780]; also known as encephaloclastic porencephaly. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Inheritance is autosomal dominant. SIMILARITY: Belongs to the type IV collagen family. & Contains 2 COL4C (collagen IV C-terminal) domains. |
| Stem Cell Type |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Store at -20ºC for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
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| Material Size | 100 µg |