| Description | |
|---|---|
| Catalogue Number | CC1019 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Human Integrin αVβ3, Triton X-100 Formulation |
| Overview | Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to aVb3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described (Belkin, 1990). |
| Alternate Names |
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| Product Information | |
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| Presentation | Purified protein in 20 mM Tris-HCI, pH 7.5, 150 mM NaCI, 2 mM MgCI2, 0.2% Triton X-100. No preservatives. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Application Notes | Electrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2. Optimal working dilutions must be determined by end user. |
| Biological Information | |
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| Concentration | 0.2 mg/mL |
| Purity | > 95% pure by gel analysis. Nonreduced 7.5% PAAG stained with Silver stain shows major bands at 92 kDa (beta3 subunit), 145 kDa (alphaV subunit) and a minor band at 170 kDa (beta3 subunit dimer). |
| Specific Activity | Functional activity confirmed by interaction of integrin alphaV/beta3 with vitronectin, von Willebrand Factor, fibrinogen, and fibronectin in RGD-dependent ELISA testing. |
| Entrez Gene Number |
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| Entrez Gene Summary | ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SIZE: 1048 amino acids; 116038 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at -70°C in undiluted aliquots. Avoid repeated freeze/ thaw cycles. |
| Packaging Information | |
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| Material Size | 50 µg |