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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, B, M, R | IHC, IH(P), IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB079-1 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-S-100 Protein Antibody, clone 15E2E2 |
| Alternate Names |
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| Background Information | S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer. Contains 0.01% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: A previous lot of this antibody was used in IP. Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques. Immunohistochemistry: A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified bovine S-100 protein |
| Epitope | Recognizes both alpha & beta S100 |
| Clone | 15E2E2 |
| Concentration | 0.5 mg/mL |
| Host | Mouse |
| Specificity | This antibody recognizes ~22 kDa (non-reduced) or 11 kDa (reduced) of S100. It reacts with human, rat, mouse, and cow (bovine). Other species are not tested. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. |
| Gene Symbol |
|
| Purification Method | The mouse IgG is purified by protein A-affinity chromatography according to isotyping. |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P23297 # Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. SIZE: 94 amino acids; 10546 Da SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. SIMILARITY: SwissProt: P23297 ## Belongs to the S-100 family. & Contains 2 EF-hand domains. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on PC12 lysates Western Blot Analysis: 1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |