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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M | ELISA, IP, WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB10005 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-ABCA1 Antibody, clone AB.H10 |
| Alternate Names |
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| Background Information | Mutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Presented as a liquid in PBS, pH 7.4 with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-ABCA1 Antibody, clone AB.H10 is validated for use in ELISA, IP, WB, IH(P) for the detection of ABCA1. |
| Key Applications |
|
| Application Notes | ELISA Immunohistochemistry (Paraffin): 5 μg/mL. Perform heat mediated antigen retrieval in sodium citrate buffer (pH 6), before commencing with IHC staining protocol. Amplification with biotin-streptavidin may be required. Immunoprecipitation Western Blot: 1 μg/mL. Predicted molecular weight: 254 kDa. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1. |
| Clone | AB.H10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognize specifically ABCA1 from human, mouse, and chicken. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangiers disease and familial high-density lipoprotein deficiency. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O95477 # cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. SIZE: 2261 amino acids; 254286 Da SUBUNIT: Interacts with MEGF10. TISSUE SPECIFICITY: Widely expressed, but most abundant in macrophages. DOMAIN: SwissProt: O95477 Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. PTM: Phosphorylation on Ser-2054 regulates phospholipid efflux. DISEASE: SwissProt: O95477 # Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. & Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux. SIMILARITY: Belongs to the ABC transporter family. ABCA subfamily. & Contains 2 ABC transporter domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |