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Millipore Detect Phospholipase A2 Using This Anti-Phospholipase A2 Antibody, Clone Ch-7 Validated For Use In Elisa & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
In 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6
Quality Level
MQ100
Applications
Application
Detect Phospholipase A2 using this Anti-Phospholipase A2 Antibody, clone CH-7 validated for use in ELISA & WB.
Key Applications
ELISA
Western Blotting
Application Notes
Immunoblot: 1:500-1:1000; antibody recognizes all isoforms, the cPLA.
Alpha (85kDa) typically migrates as 100-110kDa on reducing westerns; beta migrates as 114kDa; antibody will also recognize the sPLA at approximately 14-20kDa.
ELISA
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Recombinant cPLA2 expressed from the human cPLA2 gene.
Clone
CH-7
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Antibody reacts with cytosolic form of PLA2. Antibody will also react with beta isoform. Epitope has been localized within the first 216 aa of the N-terminal Ca++ binding domain of cPLA2
Isotype
IgG2b
Species Reactivity
Recombinant Human Protein
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NM_000928.2
Entrez Gene Summary
Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM]
Gene Symbol
PLA2G1B
MGC119834
PPLA2
PLA2
PLA2A
MGC119835
EC 3.1.1.4
Modifications
Phosphorylation
UniProt Number
P04054
UniProt Summary
FUNCTION: SwissProt: P04054 # PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. COFACTOR: Binds 1 calcium ion per subunit (By similarity). SIZE: 148 amino acids; 16360 Da SUBCELLULAR LOCATION: Secreted.SIMILARITY:SwissProt: P04054 ## Belongs to the phospholipase A2 family.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
