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Millipore Anti-Cisplatin Dna Adducts Antibody, Clone Icr4
Millipore Anti-Cisplatin Dna Adducts Antibody, Clone Icr4
List Price$528.64Your Price$528.64
Millipore Detect Cisplatin Adducts Using This Rat Monoclonal Antibody, Anti-Cisplatin Dna Adducts Antibody, Clone Icr4 Validated For Use In Dot Blot, Elisa & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Cisplatin adducts are Guanine-Guanine (GG) DNA intrastrand cross links caused by the chemical compound Cisplatin. Cisplatin is used as a chemotherapy agent that contains platinum. The crosslinking of the DNA triggers apoptosis in the effected cell. Cisplatin is particularly effective in certain cancers such as testicular cancer. Unfortunately like most chemotherapy agents Cisplatin has many side effects and cancer cells typically will develop resistance to the drug after a period of time. Clone ICR4 was formerly known as clone CP9/19.
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Cisplatin adducts using this rat monoclonal antibody, Anti-Cisplatin DNA Adducts Antibody, clone ICR4 validated for use in Dot Blot, ELISA & IHC.
Key Applications
Dot Blot
ELISA
Immunohistochemistry
Application Notes
ELISA Analysis: A representative lot from an independent laboratory detected Cisplatin DNA Adducts in ELISA (Tilby, M. J., et al. (1991). Cancer Res. 51(1):123-129.; Kothandapani, A., et al. (2011). J Biol Chem. 286(16):14564-14574.; Welters, M. J., et al. (1999). Ann Oncol. 10(1):97-103.; Strobeck, M. W., et al. (2000). Proc Natl Acad Sci USA. 97(14):7748-7753.; Kothandapani, A., et al. (2012). Exp Cell Res. 18(16):1973-1986.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cisplatin DNA Adducts in Head and Neck Squamous Cell Carcinoma tissues. (Welters, M. J., et al. (1999). Ann Oncol. 10(1):97-103.).
Biological Information
Immunogen
Cisplatin modified native DNA.
Clone
1CR4
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rat
Specificity
This antibody demonstrates specificity for cisplatin modified DNA.
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Purification Method
Protein G Purified
Product Usage Statements
Quality Assurance
Evaluated by Dot Blot in untreated and Cisplatin treated DNA from HeLa cell lysates.
Dot Blot Analysis: A 1:1,000 dilution of this antibody detected Cisplatin DNA Adducts in 1.0 and 0.5 µg of Cisplatin treated DNA from HeLa cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
