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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, ELISA, IHC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE416 |
| Description | Anti-Cisplatin DNA Adducts Antibody, clone ICR4 |
| Alternate Names |
|
| Background Information | Cisplatin adducts are Guanine-Guanine (GG) DNA intrastrand cross links caused by the chemical compound Cisplatin. Cisplatin is used as a chemotherapy agent that contains platinum. The crosslinking of the DNA triggers apoptosis in the effected cell. Cisplatin is particularly effective in certain cancers such as testicular cancer. Unfortunately like most chemotherapy agents Cisplatin has many side effects and cancer cells typically will develop resistance to the drug after a period of time. Clone ICR4 was formerly known as clone CP9/19. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cisplatin adducts using this rat monoclonal antibody, Anti-Cisplatin DNA Adducts Antibody, clone ICR4 validated for use in Dot Blot, ELISA & IHC. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot from an independent laboratory detected Cisplatin DNA Adducts in ELISA (Tilby, M. J., et al. (1991). Cancer Res. 51(1):123-129.; Kothandapani, A., et al. (2011). J Biol Chem. 286(16):14564-14574.; Welters, M. J., et al. (1999). Ann Oncol. 10(1):97-103.; Strobeck, M. W., et al. (2000). Proc Natl Acad Sci USA. 97(14):7748-7753.; Kothandapani, A., et al. (2012). Exp Cell Res. 18(16):1973-1986.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cisplatin DNA Adducts in Head and Neck Squamous Cell Carcinoma tissues. (Welters, M. J., et al. (1999). Ann Oncol. 10(1):97-103.). |
| Biological Information | |
|---|---|
| Immunogen | Cisplatin modified native DNA. |
| Clone | 1CR4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | This antibody demonstrates specificity for cisplatin modified DNA. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein G Purified |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Dot Blot in untreated and Cisplatin treated DNA from HeLa cell lysates. Dot Blot Analysis: A 1:1,000 dilution of this antibody detected Cisplatin DNA Adducts in 1.0 and 0.5 µg of Cisplatin treated DNA from HeLa cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |