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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE989 |
| Description | Anti-Homeodomain-only Protein Antibody, clone 15C1.1 |
| Alternate Names |
|
| Background Information | Homeodomain-only protein (UniProt Q9BPY8; also known as Lung cancer-associated Y protein, Not expressed in choriocarcinoma clone 1, Not expressed in choriocarcinoma protein 1, Odd homeobox 1 protein, Odd homeobox protein 1) is encoded by the HOPX (also known as CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO) gene (Gene ID 84525) in human. Human and mouse HOPX proteins share 92% identity, they contain homeobox-like domain but lack sequence motif required for DNA binding. HOPX is highly expressed in the developing heart, where its expression is dependent on the cardiac-restricted homeobox protein, Nkx2.5. HOPX physically interacts with serum responsive factor (SRF) and inhibits SRF-dependent transcription by inhibiting SRF binding to DNA and by recruiting histone deacetylase (HDAC) activity. HOPX is reported to play a critical role in the differentiation of various cells such as myoblasts, trophoblasts, keratinocytes, T cells, and lung alveolar cells. HOPX was initially reported as a differentiation marker and is expressed in various normal tissues, and HOPX expression is markedly suppressed in a subset of cancers. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Homeodomain-only Protein Antibody, clone 15C1.1 is validated for use in Western Blotting for the detection of Homeodomain-only Protein. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Homeodomain-only Protein. |
| Clone | 15C1.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with spliced isoforms 1, 3, and 4. Reactivity toward isoform 2 has not been determined. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~10 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse lung tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Homeodomain-only Protein in 10 µg of mouse lung tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |