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Millipore Anti-Beta-Mannosidase Antibody, Clone 1b3.1 Is A Highly Specific Mouse Monoclonal Antibody, That Targets Mannosidase & Has Been Tested In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Beta-mannosidase, also known as Lysosomal beta A mannosidase or Mannanase, or Mannase, and encoded by the gene MANBA/MANB1, is an exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides. Beta-mannosidase is localized to the lysosome. Beta-mannosidase is expressed in many cell types including fibroblasts, neutrophils and even neurons and glia. Defects in Beta-mannosidase may be associated with a mild disorder that affects peripheral and central nervous system myelin health and is called Mannosidoisis beta A. It is a lysosomal storage disorder that results in a mixture of clinical features from mental retardation, hearing loss and speech activities.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Beta-mannosidase Antibody, clone 1B3.1 is a highly specific mouse monoclonal antibody, that targets Mannosidase & has been tested in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Beta-mannosidase in human cerebral cortex tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Beta-mannosidase.
Clone
1B3.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_005899
Gene Symbol
MANBA
MANB1
Purification Method
Protein G Purified
UniProt Number
O00462
Molecular Weight
~80 kDa observed. The calculated molecular weight of this protein is 100 kDa. However, this protein undergoes post-translational modification, and may be observed at ~110 kDa, ~100 kDa, ~91 kDa, ~86 kDa, ~84 kDa, and ~75 kDa (Sopher, B. L., et al. (1993). Biochem J. 289(Pt 2):343-347.).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Beta-mannosidase in 10 µg of HepG2 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
