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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Ch | WB, ICC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT205 |
| Replaces | 04-1117 |
| Description | Anti-α-Tubulin Antibody, clone DM1A |
| Alternate Names |
|
| Background Information | Tubulin is one of the main components of the cytoskeleton. It is a heterodimeric structure consisting of interlocking alpha and beta chains. Like other cytoskeleton proteins, tubulin undergoes dynamic changes depending on the cellular context, which are mediated by binding and release of GDP/GTP. Tubulin proteins may also undergo post-translational modifications, and are essential to cellular transport and mitotic processes. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected α-tubulin in NIH/3T3, A431, and HeLa cells. Immunohistochemistry Analysis: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue. |
| Biological Information | |
|---|---|
| Immunogen | Native chicken brain microtubules. |
| Clone | DM1A |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react wtih Human and Mouse. Predicted to react with Chicken based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SUBUNIT STRUCTURE: Dimer of alpha and beta chains. SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton. POST-TRANSLATIONAL MODIFICATION: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively. MISCELLANEOUS: There are at least seven alpha tubulin genes (alpha-1 to alpha-6, and alpha-8), and a pseudogene (alpha-7) in chicken. SEQUENCE SIMILARITIES: Belongs to the tubulin family. |
| Molecular Weight | ~60 kDa observed. Uniprot describes a molecular weight at ~46 kDA, but can be higher due to post-translational modification. This protein has been observed at ~55 kDa in some cell lysates (Saugstad, J. A., et al. (2002). J Neurochem. 80(6):980-988.). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |