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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, ACT | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT207 |
| Description | Anti-Integrin alpha-V Antibody, clone 272-17E6 (Azide free) |
| Alternate Names |
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| Background Information | Integrin alpha-V (or CD51; vitronectin receptor subunit alpha) protein is a transmembrane receptor that mediates adhesion of cells to each other and to the extracellular matrix. Integrin receptors are activated by a variety of intracellular signals that modify its cytoplasmic domain and induce configural changes in integrin molecules thereby enabling ligand binding to the extracellular surface. An important part of this process is the binding of the actin protein, talin, to the integrin cytoplasmic domain. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing PBS without preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Integrin alpha-V Antibody, clone 272-17E6 (Azide free) detects level of Integrin alpha-V & has been published & validated for use in Flow Cytometry, Immunoprecipitation, Activity Assay. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect Integrin alpha-V in M21 cell lysates. (Mitjans, F., et al (1995). Journal of Cell Science. 108:2825-2838.) |
| Biological Information | |
|---|---|
| Immunogen | Human Placental ɑvβ3 |
| Clone | 272-17E6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. Alternative splicing results in multiple script variants. [provided by RefSeq, Feb 2009]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SUBNUNIT STRUCTURE: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. Alpha-V/beta-6 binds to foot-and-mouth disease virus (FMDV) VP1 protein and acts as a receptor for this virus (By similarity). Alpha-V/beta-6 binds to coxsackievirus A9 and coxsackievirus B1 capsid proteins and acts as a receptor for these viruses. Interacts with RAB25. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SEQUENCE SIMILARITIES: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. SEQUENCE CAUTION: The sequence BAD93131.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
| Molecular Weight | 116 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in HeLa cells. Flow Cytometry Analysis: 0.5 µg of this antibody detected Integrin alpha-V in HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |