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Genscriptcho-K1/Cre-Cbluc/Glp1r100ug; 2vials
List Price
$10,500.00
Your Price
$10,500.00
GenScript Cho-K1/Cre-Cbluc/Glp1r100ug - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00969
MFG.PART:
M00969
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably express glucagon-like peptide-1 receptor on the surface and the luciferase reporter gene incorporated into the biological signaling pathways will be activated upon the binding of GLP-1. The surface expression of GLP1R is validated by luminescence analysis. This stable cell line product is designed for cell-based functional assays of GLP-1 or analogues targeting GLP1R. |
| Applications | Functional assays for GLP1R |
Product Introduction
| Host Cell | CHO-K1 |
| Size | 2 vials of frozen cells (>1X106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability | Stable through more than 15 passages with no significant changes in assay performance or expression profile. |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Hams F-12K (Kaighns) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141C, Gibco) |
| Culture Medium | Hams F-12K (Kaighns), 10% FBS, 500 μg/ml geneticin (Cat. No. 10131-035, Gibco), 250 μg/ml Hygromycin B (Cat. No. 10687010, Gibco) |
Background
| Background | GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning. |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
| SKU | GSCRPT-M00969 |
|---|---|
| Featured | No |
| Supplier Part Number | M00969 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |