Takara PicoPLEX Gold Single Cell DNA-Seq Kit \384 Rxns

The PicoPLEX Gold Single Cell DNA-Seq Kit is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on innovative PicoPLEX technology and yields libraries with superior reproducibility from 1–5 mammalian cells or genome equivalents of gDNA as input, providing reliable results from challenging samples such as formalin-fixed single cells. Single index and unique dual index kits are available and must be purchased separately. This product is composed of four 96-reaction kits (Cat. # R300670), 384 reactions total, of PicoPLEX Gold Single Cell DNA-Seq reagents.

The kit features a streamlined workflow consisting of four steps—from input to amplified NGS libraries—that can be completed in less than four hours. The kit uses high-fidelity enzymes and optimized primers and is suitable for accurate and reproducible profiling of copy number variants (CNVs), single-nucleotide variants (SNVs), indels, and other small structural variants. The kit is compatible with single cells and purified DNA from humans, animals, and bacteria, as well as single flow-sorted chromosomes.

PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell.Cellular gDNA extracted in Step 1 is used as template for multiple cycles of quasi-random priming and linear amplification followed by exponential library amplification.

PicoPLEX Gold Single Cell DNA-Seq Kit workflow.The four-step PicoPLEX Gold Single Cell DNA-Seq workflow takes place in two tubes or plates and is completed in less than 3 hours with just 30 minutes of hands-on time.