Takara Cellartis 3i mES/iPSC Culture Medium \200 mL

Cellartis 3i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment, maintenance, and propagation of mouse embryonic stem (mES) cells. This medium contains three selective small molecule inhibitors (3i technology: ERK/MEK inhibitor, GSK3β inhibitor, and FGFR-TK inhibitor) that act to eliminate differentiation-inducing signals and promote cell proliferation, enabling the maintenance of the pluripotent ground state without the the need for additional stimulatory cytokines.

Images of mouse ES cell line ES-E14TG2a cultured in Cellartis 3i mES/iPSC Culture Medium (Panel A) or GMEM-based medium (Panel B) before (left) and after (right) alkaline phosphatase staining.

Following injection of ES cells into the blastocyte and blastocyte implantation, fully ES-cell-derived mice (Panel A, black coat) were generated within a single generation using Cellartis 3i medium. Conventional medium resulted in chimeric mice (Panel B).