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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | FC, IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1077-I |
| Replaces | 06-1077 |
| Description | Anti-IL-22RA-1 Antibody |
| Alternate Names |
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| Background Information | Interleukin-22 receptor subunit alpha-1 (UniProt Q8N6P7; also known as CRF2-9, Cytokine receptor class-II member 9, Cytokine receptor family 2 member 9, IL-22 receptor subunit alpha-1, IL-22R-alpha-1, IL-22RA1, ZcytoR11) is encoded by the IL22RA1 (also known as IL22R) gene (Gene ID 58985) in human. IL-22RA-1 is a member of the type II cytokine receptor family that complexes with IL-10RB and mediates JAK/STAT signaling. IL22 binding to heterodimer is greater than binding to IL22RA1 alone. IL-22RA-1 is expressed in colon, liver, lung, pancreas and kidney, however, no expression is observed in immune cells, such as monocytes, T-cells, and NK-cells. It is also detected in normal blood brain barrier endothelial cells as well as in multiple sclerosis lesions. Higher expression has been observed in synovial fluid cells from rheumatoid arthritis and spondyloarthropathy patients. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-IL-22RA-1 Antibody, Cat. No. 06-1077-I is validated for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Interleukin-22 receptor subunit alpha-1. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 1 µg from a representative lot detected IL-22RA-1 immunoreactivity among one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized SW480 human colon cancer cells. Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected IL-22RA-1 in human tonsil and prostate tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence within the C-terminal region of human IL-22RA-1 cytoplasmic domain. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody targets a C-terminal sequence within the cytoplasmic domain of human IL-22 receptor subunit alpha-1. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~63 kDa observed. 61.54 kDa (signal peptide a.a. 1-15 removed) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse spleen tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected IL-22RA-1 in 10 µg of mouse spleen tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |