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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-209-I |
| Description | Anti-phospho-SHC Antibody (Tyr239, Tyr240) |
| Alternate Names |
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| Background Information | SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect SHC tyr239 and tyr240 phosphorylation using this rabbit polyclonal Anti-phospho-SHC (Tyr239, Tyr240) antiboody, Cat. No. 07-209-I, validated for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a target site sequence surrounding phosphorylated Tyr239 and Tyr240 of human spliced isoform p52Shc. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Numbering of the target pTyr residues varies depending on the species and spliced isoforms. Target pTyr residues this rabbit polyclonal detects correspond to pTyr349/pTyr350 of human spliced isoform 6 and p66Shc, pTyr239/pTyr240 of human spliced isoform 7 and p52Shc, pTyr194/pTyr195 of isoform p46Shc, and pTyr135/pTyr136 of isoform 5 reported by UniProt (P29353). |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Bovine, Chicken, Mouse, Rat, Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
|
| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~52 kDa observed. 62.82/51.61/46.67/40.42/62.89/51.68 kDa (human isoform p66Shc/p52Shc/p46Shc/5/6/7), 62.61/51.41/46.47 kDa (mouse isoform p66Shc/p52Shc/p47Shc), and 51.50 kDa (rat) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected SHC tyrosine phosphorylation induction in 10 µg of lysate from EGF-stimulated HEK293 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |