Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-217-I |
| Replaces | 07-217 |
| Description | Anti-NAG-1/GDF15 |
| Alternate Names |
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| Background Information | Growth/differentiation factor 15 (UniProt: Q99988; also known as GDF-15, Macrophage inhibitory cytokine 1, MIC-1, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1, Placental TGF-beta, Placental bone morphogenetic protein, and Prostate differentiation factor) is encoded by the GDF15 (also known as MIC1, PDF, PLAB, PTGFB) gene (Gene ID: 9518) in human. NAG-1 is a member of the TGF-beta super family, however, it does not display strong homology to existing families. NAG-1 is synthesized as a 308-amino acid pro-NAG-1 monomer and then dimerizes by a specific disulfide linkage. The pro-NAG-1 dimer is then cleaved by furin-like proteases at an RXXR site, forming a 112 amino acid C-terminal dimeric protein and pro-peptide. It is expressed in placenta and is found in lower levels in prostate, kidney, and colon. Its expression is significantly increased during inflammation, injury, and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. NAG-1 expression is also shown to increase following exposure to a number of non-steroidal anti-inflammatory drugs. Induced expression of NAG-1 is shown to result in stimulation of apoptosis and inhibition of cell growth. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Growth/differentiation factor 15 using this rabbit polyclonal Anti-NAG-1/GDF15, Cat. No. 07-217-I, useing Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected NAG-1/GDF15 in 10 µg of HepG2 conditioned media. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NAG-1/GDF15 in human placenta tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal end of human NAG-1/GDF15. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody specifically detects Growth/differentiation factor 15 in human. |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 34.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in LNCap Conditioned Media. Western Blotting Analysis: 2 µg/mL of this antibody detected NAG-1/GDF15 in 10 µg of LNCap Conditioned Media. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |