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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-496-I |
| Replaces | 07-496 |
| Description | Anti-Myeloperoxidase Antibody |
| Alternate Names |
|
| Background Information | Myeloperoxidase (EC 1.11.2.2; UniProt P05164) is encoded by the MPO gene (Gene ID 4353) in human. Myeloperoxidase/MPO is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a 745 a.a. single chain precursor with a signal peptide sequence (a.a. 1-48), myeloperoxidase is subsequently cleaved into a light chain (a.a.165-278) and a heavy chain (a.a. 279-745). The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. MPO catalyzes hydrogen peroxide-dependent oxidations and the formation of hypochlorous acid that is central to the microbicidal activity of netrophils. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Myeloperoxidase Antibody, Cat. No. 07-496-I, is a highly specific rabbit polyclonal antibody that targets Myeloperoxidase and has been tested in Flow Cytometry and Western Blotting. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 0.1 µg from a representative lot detected myeloperoxidase immunoreactivity in 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized HL-60 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence within human myeloperoxidase heavy chain region. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | The immunogen sequence is present in the pro-fom and the mature forms of myeloperoxidase (without a.a. 1-48, a.a. 1-154, or a.a. 1-164), as well as the cleaved heavy chain (a.a. 279-745), but not light chain (a.a. 165-278). |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity purified. |
| UniProt Number |
|
| Molecular Weight | ~92/60 kDa (glycosylated pro-form/heavy chain) observed. 83.87/73.85/87.25 kDa (isoform H17/H14/H7 pro-form), 78.94/82.32 kDa (isoform H17/H7 with a.a. 1-48 propeptide removed), 66.45/66.45/69.83 kDa (isoform H17/H14/H7 with a.a. 1-154 removed), 65.48/65.48/68.86 kDa (isoform H17/H7 isoform H17/H14/H7 with a.a. 1-164 removed), 52.51 kDa (heavy chain; a.a. 279-745) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected myeloperoxidase heavy chain in 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |