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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-604-I |
| Replaces | 07-604 |
| Description | Anti-Rac2 Antibody |
| Alternate Names |
|
| Background Information | Ras-related C3 botulinum toxin substrate 2 (UniProt P15153; also known as GX, p21-Rac2, Small G protein) is encoded by the RAC2 gene (Gene ID 5880) in human. Rho family of small GTPases are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Rac1 and Rac2 share high sequence homology (90%) and are both expressed in the hematopoietic system, where they play crucial roles in mediating the interactions between hematopoietic stem cells (HSCs) and their microenvironment. Rac proteins transduce cell surface signaling events initiated by tyrosine kinase receptors, G protein-coupled receptors, and cell-to-cell contacts by acting as molecular switches, cycling between an inactive GDP-bound state and a GTP-bound active state. In addition to overlapping functions, Rac1 and Rac2 also mediate unique functions via interactions with specific partners. SAM50 and Metaxin1, for example, are among mitochondrial proteins identified to be specifically associated with Rac2. Rac activity inhibition is reported to effectively target primary human AML and CML cells and prevent disease initiation in various murine leukemia models. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Rac2 using this rabbit polyclonal Anti-Rac2, Cat. No. 07-604-I, validated for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Rac2 in human tonsil, human small intestine, and mouse spleen tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant full-length human Rac2 pro-form. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody was raised against recombinant full-length human Rac2 pro-form (a.a. 1-192) and therefore will recognize both the pro- and mature (a.a. 1-189) Rac2. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~21 kDa observed. 21.12/21.43 kDa (human mature/pro-form) and 21.13/21.44 kDa (mouse mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HL-60 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Rac2 in 10 µg of HL-60 human promyelocytic leukemia cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |