Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P), ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-609-I |
| Replaces | 07-609 |
| Description | Anti-Peroxiredoxin 1 |
| Alternate Names |
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| Background Information | Peroxiredoxin-1 (UniProt: Q06830; also known as EC:1.11.1.15, Natural killer cell-enhancing factor A, NKEF-A, Proliferation-associated gene protein, PAG, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2) is encoded by the PRDX1 (also known as PAGA, PAGB, TDPX2) gene (Gene ID: 5052 ) in human. Peroxiredoxins belong to a family of non-seleno peroxidases. Thus far six different mammalian isoforms have been reported and they all catalyze peroxide reduction of hydrogen peroxide, organic hydroperoxides and peroxynitrite. All peroxiredoxins share a conserved Cys residue that corresponds to the N-terminal Cys51 in mammalian peroxiredoxin-1. Peroxiredoxin 1-4 also contain an additional conserved Cys residue in the C-terminal region, Cys172. The N-terminal Cys51 is shown to be oxidized by hydrogen peroxide to form cysteine-sulfenic acid (Cys51-SOH) and reacts with Cys172-SH of the other subunit to produce an intermolecular disulfide, which can only be reduced by thioredoxin and not by glutathione or glutaredoxin. Peroxiredoxin-1 is constitutively expressed in most human cells and is induced to higher levels upon serum stimulation in cells. It is involved in redox regulation of the cell and important role in overcoming oxidative stress. Peroxiredoxin-1 also eliminates peroxides generated during drug metabolism. It is phosphorylated on Thr90 during the M-phase of cell cycle, which leads to over 80% reduction in its enzymatic activity. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Peroxiredoxin 1 Antibody, Cat. No. 07-609-I, is a highly specific rabbit polyclonal antibody that targets Peroxiredoxin 1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Peroxiredoxin 1 in HeLa cells. Immunohistochemistry Analysis: A 1:250-1:1,000 dilution from a representative lot detected Peroxiredoxin 1 in human kidney, human small intestine, and human cerebellum tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linnear peptide corresponding to 12 amino acids from the C-terminal region of human Peroxiredoxin-1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects Peroxiredoxin-1 by Western blotting and immunocytochemistry in in DU145 cells and HeLa cells, respectively. |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with various other mammalian species, including Bat, Canine, Hamster, Rat, and Rhesus Monkey. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~21 kDa observed; 22.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in DU145 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Peroxiredoxin 1 in 10 µg of DU145 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |