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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ICC | Rb | AlexaFluor®555 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1413-I-AF555 |
| Description | Anti-Interleukin-1β Antibody, Alexa Fluor® 555 Conjugate |
| Alternate Names |
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| Background Information | Interleukin-1 beta (UniProt; also known as IL-1 beta, IL-1b, IL-1beta) is encoded by the Il1b gene (Gene ID 16176) in murine species. Interleukin-1 (IL-1) is a cytokine that plays a central role in the regulation of acute and chronic inflammation. Two forms of IL-1, IL-1 and IL-1 , are transcribed from two separate genes and mediate similar, but not completely overlapping, biological effects via the membrane-bound IL-1 type 1 receptor (IL-1R1). Studies utilizing Il1a- and Il1b-knockout mice showed that IL-1 , but not IL-1 , is involved in T-cell-dependent antibody production via antigen-specific T-cell activation. IL-1 binding to the extracellular domain of IL1R1 promotes the recruitment of the IL-1 receptor accessory protein and subsequent signal transduction mediated by the cytoplasmic domains of IL-1R1 and IL1-R2. The IL-1 type 2 receptor (IL1R2) is unable to initiate signaling and only acts as a decoy receptor. Soluble receptors for IL-1 (Sil-1Rs) are produced by proteolytic cleavage of membrane-bound receptors or by alternative splicing and act as natural inhibitors by competing against membrane-bound IL1Rs for IL-1 binding. Murine IL-1 beta is produced with a propeptide sequence (a.a. 1-117), which is removed posttranslationally to yield the mature (a.a. 118-269) protein. |
| Product Information | |
|---|---|
| Format | AlexaFluor®555 |
| Presentation | Purified rabbit polyclonal antibody conjugate in buffer containing PBS with 1.5% BSA and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-Interleukin-1 Antibody, Alexa Fluor® 555 Conjugate, Cat. No. AB1413-I-AF555, is validated for use in Immunocytochemistry for the detection of Interleukin-1 . |
| Key Applications |
|
| Application Notes | The unconjugated antibody (Cat. No. AB1413-I) and Alexa Fluor® 488 conjugate (Cat. No. AB1413-I-AF488) are also available for Immunocytochemistry, Immunoprecipitation, and Western Blotting applications. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence within the C-terminal half of murine and rat mature IL-1beta. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | The unconjugated antibody (Cat. No. AB1413-I) detected both the ~17 kDa mature and ~30 kDa pro-form of murine IL-1beta. The corresponding sequence in human IL-1beta differs from that of the immunogen by only one amino acid. Immunogen sequence is not found in IL-1alpha. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Gerbil, Hamster, and Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~17/30 kDa observed. 17.39/30.93 kDa (mouse mature/pro-form), 17.32/30.64 kDa (rat mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in NIH/3T3 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IL-1beta in 4% paraformaldehyde-fixed, Triton X-100-permeabilized NIH/3T3 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |