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Millipore Anti-Vegf-A Antibody Is An Antibody Against Vegf-A For Use In Western Blotting And Immunohistochemistry (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
The human VEGFA, a 45 kDa homodimeric glycoprotein, is one of the most potent angiogenic factors involved in endothelial cell proliferation, migration, and secretion of MMPs. It also serves as an important survival factor for endothelial cells, inhibiting apoptosis. VEGFA belongs to a family that includes VEGFA, VEGFB, VEGFC, VEGFD, and placental growth factor (PLGF). In humans four prominent isoforms of VEGFA are recognized, VEGF121, VEGF165, VEGF189, and VEGF206. In addition, two other less frequent splice variants, VEGF145 and VEGF183, have also been reported. The properties of native VEGF are very similar to VEGF165. Of all the different isoforms of VEGF, the VEGF165 has the highest bioavailability and biological potency. VEGFA action is mediated by VEGF receptor-1 (VEGFR-1) and VEGFR-2. VEGFR-1 (180 kDa, Flt-1) expression is upregulated by hypoxic conditions in the inner core of tumor mass. VEGFR-2 (200 kDa, KDR) is a major mediator of mitogenic and angiogenic permeability enhancing effects of VEGF. It is also reported to mediate survival signals in endothelial cells.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-VEGF-A Antibody is an antibody against VEGF-A for use in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected VEGF-A in mouse kidney, mouse cerebellum, mouse spleen, and rat kidney tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to mouse VEGF-A near the N-terminus.
Epitope
Near N-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001273985
Gene Symbol
Vegfa
Vegf
Purification Method
Affinity
UniProt Number
Q00731
Molecular Weight
~27 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse lung tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected VEGF-A in 10 µg of mouse lung tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
