Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, IF | Ch | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB356481 |
| Description | Anti-mCherry |
| Background Information | mCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but it produces a red rather than a green fluorescence. mCherry is a monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, polyclonal was raised in chicken egg and the antibody preparation was dialyzed against phosphate buffered saline. This antibody recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et Al. (2004). Nat. Biotechnol. 22(12):1567-72; Gross, LA et Al. (2000). Proc. Natl. Acad. Sci. USA. 97(22):11990-95). |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Chicken polyclonal antibody in PBS with 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect mCherry protein using this chicken polyclonal anti-mCherry Antibody, Cat. No. AB356481. It has been tested in Immunofluorescence and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. (Courtesy of Dr Gerry Shaw). Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. (Courtesy of Dr Gerry Shaw). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full length recombinant mCherry from Discosoma sp (Mushroom anemone). |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Chicken |
| Specificity | This polyclonal antibody detects mCherry in HEK293 cells tranfected with pFin-mCherry. |
| Isotype | IgY |
| Species Reactivity |
|
| Species Reactivity Note | All |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| Molecular Weight | ~32 kDa observed; 25.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 transfected with pFin-EF1-mCherry vector. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected mCherry in 10 µg of HEK293 cells transfected with pFin-EF1-mCherry vector. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |