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Millipore Detect Mcherry Protein Using This Chicken Polyclonal Anti-Mcherry Antibody, Cat. No. Ab356481. It Has Been Tested In Immunofluorescence And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
mCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but it produces a red rather than a green fluorescence. mCherry is a monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, polyclonal was raised in chicken egg and the antibody preparation was dialyzed against phosphate buffered saline. This antibody recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72; Gross, LA et al. (2000). Proc. Natl. Acad. Sci. USA. 97(22):11990-95).
Product Information
Format
Unpurified
Presentation
Chicken polyclonal antibody in PBS with 0.02% sodium azide.
Quality Level
MQ100
Applications
Application
Detect mCherry protein using this chicken polyclonal anti-mCherry Antibody, Cat. No. AB356481. It has been tested in Immunofluorescence and Western Blotting.
Key Applications
Western Blotting
Immunofluorescence
Application Notes
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. (Courtesy of Dr Gerry Shaw).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. (Courtesy of Dr Gerry Shaw).
Biological Information
Immunogen
His-tagged full length recombinant mCherry from Discosoma sp (Mushroom anemone).
Epitope
unknown
Concentration
Please refer to lot specific datasheet.
Host
Chicken
Specificity
This polyclonal antibody detects mCherry in HEK293 cells tranfected with pFin-mCherry.
Isotype
IgY
Species Reactivity
All
Species Reactivity Note
All
Antibody Type
Polyclonal Antibody
Gene Symbol
mCherry
Purification Method
Unpurified
UniProt Number
Q9U6Y8
Molecular Weight
~32 kDa observed; 25.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 transfected with pFin-EF1-mCherry vector.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected mCherry in 10 µg of HEK293 cells transfected with pFin-EF1-mCherry vector.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
