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Millipore Detect Beta-Lactamase Using This Rabbit Anti-Beta Lactamase Polyclonal Antibody, Cat. No. Ab3738-I, Validated For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Beta-lactamase TEM (EC 3.5.2.6; UniProt P62593; also known as IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2) is encoded by the bla (also known as blaT-3, blaT-4, blaT-5, blaT-6) gene (Gene ID 3244915) in E. coli. TEM-1 belongs to the class-A beta-lactamase family. Beta-lactamases produced by bacteria provide resistance to beta-lactam antibiotics, such as penicillins and cephamycins by breaking open the beta-lactam ring. Over 200 beta-lactamases have been reported that are classified into four main groups (A, B, C, and D) and eight subgroups according to their functional and structural characteristics. The TEM-1 is predominant plasmid-mediated beta-lactamase of gram-negative rods. Six different nucleotide sequences, called blaTEM-1a, blaTEM-1b, blaTEM-1c, blaTEM-1d, blaTEM-1e, and blaTEM-1f, have been described to codify the same TEM-1 beta-lactamase. Ref.: Briñas, L., et al. (2002). Antimicrob. Agents Chemother. 46, 103156-103163.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect beta-lactamase using this rabbit Anti-Beta Lactamase polyclonal antibody, Cat. No. AB3738-I, validated for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
GST-tagged full-length E. coli beta-lactamase, including the signal peptide sequence.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This polyclonal antibody detects E. coli beta-lactamase.
Species Reactivity
All
Species Reactivity Note
E. Coli.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_957565
Gene Symbol
bla
blaT-3
blaT-4
blaT-5
blaT-6
Purification Method
Affinity purified.
UniProt Number
P62593
Molecular Weight
31.52/28.91 kDa (with/without signal peptide a.a. 1-23) calculated.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in lasate from beta-lactamase-transformed E.coli.
Western Blotting Analysis: A 1:100,000 dilution of this antibody detected GST-tagged full-length E. coli beta-lactamase in 0.5 µg of lysate from transformed E. coli.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
