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Millipore This Anti-Sox9 Antibody, Alexa Fluor® 488 Conjugate Is Validated For Use In Immunocytochemistry For The Detection Of Sox9. - Mill (Additional S&H or Hazmat Fees May Apply)
Transcription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences that encode for the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified.
Product Information
Format
AlexaFluor®488
Presentation
Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.
Key Applications
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sox9 in HepG2 cells. The unconjugated version (Cat. No. AB5535) is also shown to be suitable for Western blotting and immunohistochemistry applications.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human Sox9.
Epitope
C-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Rat
Human
Mouse
Chicken
Species Reactivity Note
Rat, Human, Mouse, Chicken. Predicted to react with Bovine, Feline, Horse based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_000337
Gene Symbol
SOX9
Purification Method
Affinity Purfied
UniProt Number
P48436
Molecular Weight
55-65 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sox9 in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
