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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P), IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9094-I |
| Replaces | AB9094 |
| Description | Anti-Sodium Pump Subunit alpha-2 Antibody |
| Alternate Names |
|
| Background Information | Sodium/potassium-transporting ATPase subunit alpha-2 (EC 3.6.3.9; UniProt P06686; also known as Na+/K+ -ATPase alpha 2 subunit, Na(+)/K(+) ATPase alpha(+) subunit, Sodium pump subunit alpha-2) is encoded by the Atp1a2 (also known as RATATPA2) gene (Gene ID 24212) in rat. Mammalian Na+/K+ ATPase is a heteromultimeric complex composed of alpha, beta, and gamma subunits. The alpha subunit catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ across the plasma membrane. The alpha subunit contains ten transmembrane domains with both its N- and C-termini in the cytoplasm. More than 50 mutations have been identified affecting the human ATP1A2 and ATP1A3 genes that are known to cause rapid-onset Dystonia Parkinsonism, familial hemiplegic migraine, alternating hemiplegia of childhood, and variants of familial hemiplegic migraine with neurological complications including seizures and various mood disorders. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Sodium Pump Subunit alpha-2 using this Anti-Sodium Pump Subunit alpha-2 Antibody validated for use in Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis (IHC): A representative lot detected Sodium Pump Subunit alpha-2 in human (1:1,000) and rat (1:250) cerebellum tissue sections. Immunoprecipitation Analysis (IP): 10 ug from a representative lot immunoprecipitated Sodium Pump Subunit alpha-2 in 500 µg of rat brain tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the second cytoplasmic domain of rat Sodium Pump Subunit alpha-2. |
| Epitope | Second cytoplasmic domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Reacts with Sodium Pump (Na+ pump (Na+-K+-ATPase)) alpha 2 isoform. The antibody does not cross react with other Na+-K+-ATPase alpha isoforms. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Bovine, Porcine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~100 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: A 1:4,000 dilution of this antibody detected Sodium Pump Subunit alpha-2 in 10 µg of rat brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |