Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Semi-Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC1434 |
| Description | Anti-UVRAG-FS mutant |
| Alternate Names |
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| Background Information | UV radiation resistance-associated gene protein (UniProt: Q9P2Y5; also known as p63) is encoded by UVRAG gene (Gene ID: 7405) in human. UVRAG is a key autophagic tumor suppressor that is shown to be mutated in several common human cancers. UVRAG as a critical regulator of intracellular membrane trafficking, autophagy, and chromosomal stability. It contains four functional domains, a proline-rich domain, a lipid-binding C2 domain, a Beclin1-binding coiled-coil domain (CCD) and a C-terminal domain. UVRAG is truncated by a frameshift (FS) mutation in colorectal cancer cells. UVRAG-FS is shown to abolish the normal functions of UVRAG, including autophagy, in a dominant-negative manner. Ectopic expression of UVRAG-FS in both embryonic stem cells and cancer cells results in extensive centrosome amplification and concomitant aneuploidy. UVRAG-FS can also enhance the sensitivity to anticancer agents, such as 5-fluorouracil (5-FU), oxaliplatin, and irinotecan. Expression of UVRAG-FS is reported to trigger colorectal cancer metastatic spread through Rac1 activation and epithelial-to-mesenchymal transition, independently of autophagy. UVRAG-FS metastatic tumors display higher levels of p62 than primary tumors, which indicates suppressed autophagy. (Ref.: He, S., et Al. (2015). Nat Commun. 6: 7839). |
| Product Information | |
|---|---|
| Format | Semi-Purified |
| Presentation | Rabbit polyclonal antibody 1XPBS with 0.05% azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-UVRAG-FS mutant, Cat. No. ABC1434, detects FS mutant UV radiation resistance-associated gene protein. Validated for use in Immunoprecipitation and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected UV radiation resistance-associated gene protein in Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines (Data courtesy of Dr Liang Laboratory). Western Blotting Analysis: A representative lot detected Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines. (He, S., et. Al. (2015). Nat Commun. 6:7839). Immunoprecipitation Analysis: A representative lot detected Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines. (He, S., et. Al. (2015). Nat Commun. 6:7839). |
| Biological Information | |
|---|---|
| Immunogen | A frameshift (FS)-UVRAG derived linear peptide. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody targets an internal sequence in the N-terminal half and detects UVRAG-FS by Western blotting. |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Ammonium Sulfate |
| UniProt Number |
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| Molecular Weight | ~32 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in WT/ Mutant UVRAG-FS lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UV radiation resistance-associated gene protein in Wild-type (WT) and FS mutant UVRAG protein expression in microsatellite stable (MSS) and microsatellite instable (MSI) colorectal cancer cell lines. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |