| Description | |
|---|---|
| Catalogue Number | ABC503 |
| Description | Anti-Cathepsin S |
| Alternate Names |
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| Background Information | Cathepsin S (Uniprot: P25774; EC: 3.4.22.27) is encoded by the CTSS gene (Gene ID: 1520) in human. Cathepsin S is a lysosomal thiol protease that removes invariant chain from MHC class II molecules. It displays higher activity on ZVal-val-Arg-/-Xaa compounds and less activity on Z-Phe-Arg-/-NHMec. Cathepsin S is secreted with a signal (aa 1-16) and a pro-peptide (aa 17-114) sequence that are cleaved to produce active enzyme (aa 115-331). Cathepsin S is involved in antigen presentation, general protein catabolism, and tumor progression. Because of its redistribution or increased levels in human and animal tumors, cathepsin S plays a role in invasion and metastasis. Inhibition of cathepsin S is shown to induce autophagy in tumor cells through EGFR signaling pathway by facilitating EGF-mediated EGFR degradation. Cathepsin S inhibition is shown to delay EGFR degradation and cause EGFR accumulation in the late endosomes at the perinuclear region. (Ref.: Beers, C et al. (2005). J. Immunol. 174(3): 1205-1212; Huang CC et al. (2016). Sci. Rep. 6:29256). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cathepsin S. Cat. No. ABC503, is a rabbit polyclonal antibody that detects Cathepsin S and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
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| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cathepsin S in human lymphoma, human small intestine, and human pancreas tissues. |
| Biological Information | |
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| Immunogen | KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human cathepsin S. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects cathepsin S in human cells. It targets an epitope within 21 amino acids from the internal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~37 kDa observed; 37.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in human testis tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected Cathepsin S in 10 µg of human testis tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 µL |