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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1332 |
| Description | Anti-monomethyl Histone H2A (Gln104) |
| Alternate Names |
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| Background Information | Histone H2A type 1 (UniProt P0C0S8; also known as H2A.1, Histone H2A/p) is encoded by the HIST1H2AG (also known as H2AFP, HIST1H2AI, H2AFC, HIST1H2AK, H2AFD, HIST1H2AL, H2AFI, HIST1H2AM, H2AFN) gene (Gene ID 8969) in human. Histone H2A is one of four components of the core nucleosome structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones. Histones undergo a number of post-translational modifications in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by Tip60 enzyme, which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues, which mark mitosis and gene repression and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. Monoubiquitination of Lys-120 (H2AK119Ub) by RING1, TRIM27 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-monomethyl Histone H2A (Gln104) Antibody, Cat. No. ABE1332, is a highly specific rabbit polyclonal antibody that targets histone H2A Gln104 monomethylation (Q104me) and has been tested by histone peptide array Dot Blot. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human histone H2A target region sequence containing monomethylated Gln104 (Q104me). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Specificity of this polyclonal antibody was verified by dot blot analysis. This polyclonal antibody specifically detected peptide corresponding to histone H2A target region sequence with monomethylated Gln104 (Q104me), but not H2A peptides without Q104me modification, nor peptides derived from histone H1, H2A.Z, H2B, H3, H3.3, or H4 sequences. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | 13.96 kDa (Met1 removed) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Dot Blot Analysis of histone peptide array. Dot Blot Analysis: A 1:200 dilution of this antibody detected 4-40 ng of histone H2A peptide containing monomethylated Gln104 (Q104me). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |