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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1400 |
| Description | Anti-TFE3 |
| Alternate Names |
|
| Background Information | Transcription factor E3 (UniProt P19532; also known as bHLHe33, Class E basic helix-loop-helix protein 33, TFE3) is encoded by the TFE3 (also known as BHLHE33) gene (Gene ID 7030) in human. TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. It specifically recognizes and binds E-box sequences (5-CANNTG-3). TFE3 is ubiquitously expressed and can directly associate with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. In association with TFEB, TFE3 activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and in thymus-dependent humoral immunity. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB is expressed at low levels in the embryo, but is expressed at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization. It has been shown that the exit from pluripotency is gated by intracellular redistribution of TFE3. Chromosomal aberrations involving TFE3 have been reported in patients with papillary renal cell carcinoma. Ref.: Betschinger, J., et al. (2013). Cell 153, 335-347. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TFE3, Cat. No. ABE1400, is a highly specific rabbit polyclonal antibody that targets transcription factor E3 (TFE3) and has been tested in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected TFE3 in 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeablized MEF-1 mouse embryonic fibrolasts. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a leucine zipper region sequence of human TFE3. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody targets the human TFE3 leucine zipper region sequence. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~75 kDa observed. Target band size appears larger than the calculated molecular weight of 61.52 kDa due to posttranslational modification. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH/3T3 nuclear extract. Western Blotting Analysis: 0.1 µg/mL of this antibody detected TFE3 in 10 µg of NIH/3T3 nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |