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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, DB, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1847 |
| Description | Anti-phospho Mis18-binding protein 1 (Thr702) Antibody |
| Alternate Names |
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| Background Information | Mis18-binding protein 1 (UniProt Q6P0N0; also known as hsKNL-2, Kinetochore null 2 homolog, Kinetochore-associated protein KNL-2 homolog, P243, Putative protein p243 which interacts with transcription factor Sp1) is encoded by the MIS18BP1 (also known as C14orf106, KIAA1903, KNL2, M18BP1) gene (Gene ID 55320) in human. Mis18-binding protein 1 is a component of the Mis18 complex (Mis18α, Mis18β, and M18BP1/KNL2) that plays a role in mediating cell cycle-dependent deposition/incorporation of the histone H3 variant CENP-A at the centromeres. CENP-A incorporation is carried out by at least two sets of events, the Mis18 complex must first localize to centromeres at the onset of anaphase, HJURP-mediated CENP-A deposition then occurs during G1. Polo-like kinase 1 (Plk1) is shown to phosphorylate M18BP1 on Thr702 and plays an important role in promoting Mis18 complex centromere localization. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho Mis18-binding protein 1 (Thr702) Antibody is an antibody against phospho Mis18-binding protein 1 for use in Immunocytochemistry, DOT Blot, Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected phospho Mis18-binding protein 1 (Thr702) in HeLa cells. DOT Blot Analysis: 1 µg/mL from a representative lot detected the immunogen peptide, but not the corresponding non-phosphorylated sequence, by DOT blot analysis. Immunocytochemistry Analysis: A representative lot detected Thr702 phosphorylated Mis18-binding protein 1/M18BP1 localized at the centromeres in HeLa cells (McKinley, K.L., and Cheeseman, I.M. (2014). Cell. 158(2):397-411). Western Blotting Analysis: A representative lot detected in vitro phosphorylation of recombinant Mis18-binding protein 1/M18BP1 on Thr702 by recombinant polo-like kinase 1 (Plk1) (McKinley, K.L., and Cheeseman, I.M. (2014). Cell. 158(2):397-411). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to a human M18BP1 sequence containing phosphorylated Thr702. |
| Epitope | M18BP1 pThr702 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Specifically reacts with the immunogen peptide, but not the corresponding non-phosphorylated sequence, by DOT blot analysis. Phosphorylation site is not present in the spliced isoform 2 of human Mis18-binding protein 1/M18BP1. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | 129 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected phospho Mis18-binding protein 1 (Thr702) in A431 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |