Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, ChIP, WB | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1868 |
| Description | Anti-TDRD3 |
| Alternate Names |
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| Background Information | Tudor domain-containing protein 3 (UniProt: Q9H7E2; also known as TDRD3) is encoded by the TDRD3 gene (Gene ID: 81550) in human. TDRD3 belongs to an evolutionarily conserved family of 12 proteins (TDRD1-TDRD12) that contain one or more Tudor domains, which recognize symmetric dimethylarginine (sDMA) residues with the exception of TDRD3, which associates selectively with asymmetric dimethylarginine. TDRD3, in addition to its Tudor domain, contains a putative nucleic acid recognition motif and an ubiquitin-associated domain. Tudor domains are highly conserved throughout evolution: they can be found in virtually all organisms, from bacteria to mammals. TDRD3 has been reported in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. It is found both in the nucleus and in cytoplasm. In nucleus, it acts as a coactivator and recognizes and binds asymmetric dimethylation on the core histone tails. The Tudor domain specifically recognizes and binds asymmetric dimethylation of histone H3 Arg-17 (H3R17me2a) and histones H4 Arg-3, 2 tags for epigenetic transcriptional activation. In cytoplasm, it may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins. TDRD3 is reported to act as a positive regulator of c-MYC gene transcription by recruiting topoisomerase IIIB (TOP3B) to the c-MYC gene promoter region via H4R3me2a recognition. Overexpression of TDRD3 gene has a strong predictive value for poor prognosis of estrogen receptor-negative breast cancers. (Ref.: Goulet, I et al. (2008). Hum. Mol. Genet. 17(19):3055-3074; Sikorsky, T et al. (2012). Nucleic Acids Res. 40(22):11748-55). |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TDRD3, Cat. No. ABE1868, is a highly specific rabbit polyclonal antibody that targets Tudor domain-containing protein 3 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected TDRD3 in 20 µL of MCF7 cell lysate. Immunoprecipitation Analysis: A representative lot detected TDRD3 in Immunoprecipitation applications (Yang, Y., et. al. (2014). Mol Cell. 53(3):484-97). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected TDRD3 in Chromatin Immunoprecipitation applications (Yang, Y., et. al. (2014). Mol Cell. 53(3):484-97;Yang, Y., et. al. (2010). Mol Cell. 40(6):1016-23). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 258 amino acids from the C-terminal half of human Tudor domain-containing protein 3. |
| Epitope | domain of: |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects the Tudor domain containing protein 3 in human. It targets an epitope within 258 amino acids in the C-terminal half. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~85 kDa observed; 73.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunoprecipitation in MCF7 cell lysate. Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated TDRD3 in 500 µg of MCF7 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |