Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IF, WB, ChIP, ICC | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1958 |
| Description | Anti-Zic1/2 |
| Alternate Names |
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| Background Information | Zinc finger protein ZIC 1 (UniProt: P46684; also known as Zinc finger protein of the cerebellum 1) is encoded by the Zic 1 (also known as Zic) gene (Gene ID: 22771) in murine species. Zic 1 is a member of the GLI C2H2-type zinc-finger protein family. Zic 1 is expressed in the central nervous system and in osteoblasts. A high level expression is seen in the cerebellum, while a low level expression is seen in the olfactory bulb, diencephalon, and brainstem. It acts as a transcriptional activator that is involved in neurogenesis and plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. The C2H2-type 3, 4 and 5 zinc finger domains of Zic 1 are considered to be essential for transcription activation and for Zic 1 interaction with MyoD family inhibitor domain-containing (MDFIC) protein. Zic 1 is also shown to be involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). It plays a role in the regulation of MF axon pathway choice and promotes MF migration towards ipsilaterally-located cerebellar territories. |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Zic1/2, Cat. No. ABE1958, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein ZIC 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Zic1/2 in mouse cerebellum (Frank, C.L., et. Al. (2015). Nat Neurosci. 18(5):647-56). Immunofluorescence Analysis: A representative lot detected Zic1/2 in postmitotic granule cells (Kim, J.Y., et. Al. (2003). Dev Biol. 263(1):50-66) and adult mouse cerebellum (Koirala, S., et. Al. (2009). J Neurosci. 29(23):7439-49). Immunocytochemistry Analysis: A representative lot detected Zic1/2 in Immunocytochemistry applications (Kenney, A.M., et. Al. (2000). Mol Cell Biol. 20(23):9055-67). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 20 amino acids from the C-terminal end of mouse Zic 1 protein. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects Zic1 protein in mouse and human. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~53 kDa observed; 48.33 kDa calculated for Zic1; 54.95 kDa calculated for Zic2. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human cerebellum tissue lysate. Western Blotting Analysis: 1:500 dilution of this antibody detected Zic1/2 in 10 µg of human cerebellum tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |