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Millipore Anti-Borealin Antibody, Cat. No. Abe1961, Is A Highly Specific Rabbit Polyclonal Antibody That Targets Borealin And Has Been Tested In Immunofluorescence, Immunoprecipitation, - Mill (Additional S&H or Hazmat Fees May Apply)
Pluripotent embryonic stem cell-related gene 3 protein
Background Information
Borealin (UniProt: Q53HL2; also known as Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein) is encoded by the CDCA8 (also known as PESCRG3) gene (Gene ID: 55143) in human. Borealin is a novel 31-kDa chromosomal passenger protein that is conserved among all vertebrates. It is a member of the chromosomal passenger holocomplex along with Aurora B, INCENP, and survivin. This complex play a vital role at the centromere to ensure proper chromosome alignment and segregation. Borealin is required for stability of the bipolar mitotic spindle and for kinetochore error correction. Borealin levels are reported to increase during G2/M phase and are reduced following exit from the M phase of cell cycle. Depletion of Borealin by RNAi is shown to retard mitotic progression and cause spindle T misattachment and abnormalities. Borealin-depleted cells are shown to align their chromosomes to a near normal metaphase plate, but they abruptly undergo multipolar anaphases.
Product Information
Format
Serum
Presentation
Rabbit polyclonal antibody serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Borealin Antibody, Cat. No. ABE1961, is a highly specific rabbit polyclonal antibody that targets Borealin and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
Western Blotting
Immunofluorescence
Immunoprecipitation
Application Notes
Immunofluorescence Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
Western Blotting Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
Immunoprecipitation Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
Biological Information
Immunogen
A His-tagged full length human recombinant Borealin.
Epitope
unknown
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This polyclonal antibody detects Borealin in nocodozole treated HeLa cells.
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_060571
Gene Symbol
CDCA8
PESCRG3
Purification Method
Unpurified
UniProt Number
Q53HL2
Molecular Weight
~36 kDa observed; 31.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Hela cell lysate treated with Nocodozole.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Borealin in 10 µg of Hela cell lysate treated with Nocodozole.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
