Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Mk, R, H | IF, ICC, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2614 |
| Description | Anti-ATF5 |
| Alternate Names |
|
| Background Information | Cyclic AMP-dependent transcription factor ATF-5 (UniProt: also known as cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx) is encoded by the ATF5 (also known as ATFX) gene (Gene ID: 22809) in human. ATF5 belongs to the ATF/CREB family of transcription factors that is widely expressed. It has been implicated in multiple biological processes, such as regulation of cell proliferation and differentiation, cell stress response and survival. ATF5 can either stimulate or repress gene transcription through binding of different DNA regulatory elements such as CRE (cAMP response element), ARE (ATF5 regulatory element), and AARE (amino acid response element). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the pericentriolar material (PCM). ATF5 depletion is reported to block PCM accumulation at the centrosome and cause fragmentation of centrioles, which contributes to the formation of multi-polar mitotic spindles and genomic instability. ATF5 homeostasis is essential for the function of the centrosome and for the global microtubule organization. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ATF5, Cat. No. ABE2614, is a highly specific rabbit polyclonal antibody that targets Cyclic AMP-dependent transcription factor ATF-5 and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected ATF5 in 10 µg of C6 cell lysate. Western Blotting Analysis: A representative lot detected ATF5 in Western Blot applications. (Madarampalli, B., et. al. (2015). Cell. 162(3):580-92). Immunofluorescence Analysis: A representative lot detected ATF5 in Immunofluorescence applications. (Madarampalli, B., et. al. (2015). Cell. 162(3):580-92). Western Blotting Analysis: A representative lot detected ATF5 in transfected Hela Cells (Courtesy of Dr. David X. Liu from Washington State University). Immunofluorescence Analysis: A representative lot detected ATF5 in Cos-7, Hela, and C6 cells (Courtesy of Dr. David X. Liu from Washington State University). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 18 amino acids from the N-terminal half of human Cyclic AMP-dependent transcription factor ATF-5. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody targets a sequence in the N-terminal region and detects ATF5 by immunocytochemstry. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Monkey, Rat |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| Molecular Weight | ~32 kDa observed; 30.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in COS-7 cells. Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected ATF5 in COS-7 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |