The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Wtap, Cat. No. Abe555, Is A Highly Specific Rabbit Polyclonal Antibody That Targets Wtap And Has Been Tested In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Pre-mRNA-splicing regulator WTAP (UniProt Q15007; also known as Female-lethal (2)D homolog, hFL(2)D, Wilms tumor 1-associating protein, WT1-associated protein) is encoded by the WTAP (also known as KIAA0105) gene (Gene ID 9589) in human. WTAP is an ubiquitously expressed nuclear protein that is localized throughout the nucleoplasm and in speckles and partially co-localizes with splicing factors. A mouse homolog has also been reported that shares 96% sequence identity. WTAP regulates G2/M cell-cycle transition by binding to the 3 UTR of CCNA2, which enhances its stability. WTAP knockdown is shown to induce G2 accumulation, which is partially rescued by adenoviral overexpression of cyclin A2. WTAP-null mice exhibit proliferative failure with early embryonic death. WTAP is also reported to be an oncogenic protein in acute myeloid leukemia (AML) and its increased expression has been linked to chemoresistance in AML. Ref: Horiuchi, K., et al. (2006), Proc. Natl. Acad. Sci. USA 103, 17278-17283. Bansal, H., et al. (2014). Leukemia 28, 1171-1174.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-WTAP, Cat. No. ABE555, is a highly specific rabbit polyclonal antibody that targets WTAP and has been tested in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human WTAP.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This polyclonal antibody targets an internal sequence within the N-terminal half of human WTAP.
Species Reactivity
Mouse
Human
Rat
Species Reactivity Note
Human, Mouse, Rat.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001257460
Gene Symbol
WTAP
KIAA0105
Purification Method
Affinity purified.
UniProt Number
Q15007
Molecular Weight
~52 observed. 44.24/17.80 kDa (isoform 1/2 calculated). Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A431.
Western Blotting Analysis: A 1:250 dilution of this antibody detected WTAP in 10 µg of A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
