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RPB3 is one of 12 subunits that make up the complete RNA polymerase, Pol II, the primary RNA polymerase in cellular metabolism. RPB3 is part of the core element of Pol II complex. RPB3 has a central large cleft and the clamp element that moves to open and close the cleft during RNA synthesis. RPB3 naturally localizes to the nucleus and expressed in all cell types and tissues. RPB3 also plays critical roles in RNA splicing and capping, key items of RNA function.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-RPB3 Antibody is an antibody against RPB3 for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human RPB3 near the Near C-terminus.
Epitope
Near C-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Rat
Mouse
Species Reactivity Note
Human, Rat, Mouse. Predicted to react with Bovine based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
P19387
Gene Symbol
POLR2C
A-152E5.7
Purification Method
Affinity Purfied
UniProt Number
P19387
Molecular Weight
~35 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected RPB3 in 10 µg of HUVEC cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
