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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABF1069 |
| Description | Anti-TOE1 (Ab-86) |
| Alternate Names |
|
| Background Information | Target of EGR1 protein 1 (UniProt Q96GM8) is encoded by the TOE1 gene (Gene ID 114034) in human. Originally characterized and thereby named as a downstream target of the immediate early gene Egr1, TOE1 is a nuclear protein localized primarily in nucleoli and Cajal bodies. TOE1 overexpression causes an upregulation of the cyclin-dependent kinase inhibitor p21 and cell cycle arrest at G2/M. TOE1 also interacts wtih p53 C-terminal tetramerization domain and enhances p53 transcriptional activity. In addtion, TOE1 exhibits inhibitory activity against HIV-1 replication and evidence indicates TOE1 interaction with the viral specific transactivator response element as part of the inhibitory mechanism. TOE1 is upregulated and secreted by activated CD8(+) T lymphocytes, and TOE1 can be proeolytically cleaved by granzyme B at D328 and D363, generating a 35-amino acid (a.a. 329-363) fragment containing the nuclear localization signal/NLS (a.a.335-347). The 35-a.a. fragment retains both the antiviral potency and cell-penetrating capability. Human TOE1 is produced as a 510-a.a. protein, containing a C3H1-type Zinc finger (a.a. 294-322) and anuclear localization signal/NLS (a.a.335-347). The initiation methionine is removed posttranslationally. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-TOE1 (Ab-86) Antibody, Cat. No. ABF1069 is validated for use in Immunoprecipitation and Western Blotting for the detection of TOE1. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 1 µL from a representative lot immunoprecipitated TOE1 from 20 µg of HEK293T cell lysate (Courtesy of Dr. Ian de Belle). Western Blotting Analysis: A representative lot detected FLAG-tagged wild-type or mutant TOE1 with D328A, D363A/D373A, or D387A point mutions following their immunoprecipitation by an anti-FLAG antibody (Sperandio, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(26):E3392-E3401). |
| Biological Information | |
|---|---|
| Immunogen | Conjugated linear peptide corresponding to the C-terminal end sequence of human TOE1. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody was raised against the C-terminal end sequence present in both spliced isoforms of human TOE1 reported by UniProt (Q96GM8). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~58 kDa observed. 56.42/47.76 kDa (isoform 1/2) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TOE1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |