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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1455 |
| Description | Anti-NKX6.2 |
| Alternate Names |
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| Background Information | Homeobox protein Nkx-6.2 (UniProt Q9C056; also known as Homeobox protein NK-6 homolog B) is encoded by the NKX6-2 (also known as GTX, NKX6B) gene (Gene ID 84504) in human. Mammalian NKX gene products and their orthologs in other species are known to regulators of neuronal cell fate. Both the ventral nervous system defective (vnd) gene product in Drosophila and murine NKX proteins in spinal cord direct progenitors to adopt specific ventral neural identities. In mice, NKX6.2 is expressed in a small subset of ventral neural progenitors in a restricted spatial pattern at the border between the medial and caudal ganglionic eminence (MGE and CGE). NKX6,2 is required for specification of V1 interneurons in the spinal cord and progenitors are found to adopt a more dorsal V0 identity in the absence of NKX6,2. In addition, NKX6.2-positive brain region is shown to give rise to both parvalbumin (PV) and somatostatin (SST) cortical interneurons and in particular be enriched in a subpopulation of SST cortical interneurons that coexpress calretinin (CR). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NKX6.2, Cat. No. ABN1455, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NKX6.2 in human cerebral cortex and kidney tissue sections. Western Blotting Analysis: 1 µg/mL from a representative lot detected Myc-DDK-tagged human NKX6.2 in 10 µg of lysate from transfected HEK293T cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human/mouse/rat NKX6.2. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detected the exogenously expressed human NKX6.2 in transfected HEK293T cells, while no immunoreactive bands were detected in lysates from untransfected cells. The immunogen sequence lies within the region C-terminal to the Homeobox and is 100% conserved among human, mouse, and rat species. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse, Rat, Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~29 kDa observed. 29.26 kDa (human) and 29.36 kDa (mouse and rat) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human pancreas tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected NKX6.2 in human pancreas tissue section. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |