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Millipore Anti-P47-Phox Antibody Is An Antibody Against P47-Phox For Use In Western Blotting, Immunohistochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Neutrophil cytosol factor 1 (UniProt Q09014), also known as 47 kDa neutrophil oxidase factor, NADPH oxidase subunit, Ncf-1, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, p47phox) is encoded by the Ncf1 gene in murine species. Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors, including p47-phox, p67-phox, p40-phox and Rac. In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight cytosolic complex. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-p47-phox Antibody is an antibody against p47-phox for use in Western Blotting, Immunohistochemistry.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p47-phox in mouse cerebral cortex and mouse small intestine.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of mouse p47-phox.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Mouse
Human
Species Reactivity Note
Mouse, Human. Predicted to react with Rat based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001272966
Gene Symbol
Ncf1
Purification Method
Affinity Purfied
UniProt Number
Q09014
Molecular Weight
~47 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected p47-phox in 10 µg of Raji cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
