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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | IHC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1643 |
| Description | Anti-APP Antibody, AEP-cleaved (373N) |
| Alternate Names |
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| Background Information | Amyloid beta A4 protein (UniProt P05067; also known as ABPP, Alzheimer disease amyloid protein, Amyloid precursor protein, APP, APPI, Cerebral vascular amyloid peptide, CVAP, PN-II, PreA4, Protease nexin-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimers disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antibody serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-APP, AEP-cleaved (373N), Cat. No. ABN1643, is a highly specific rabbit polyclonal antibody that targets the N-terminal fragment of AEP-cleaved APP at residue 373 (373N) and has been tested in Immunohistochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected APP N-terminal fragment 373N immunoreactivity in formalin-fixed, paraffin-embedded 5XFAD familial AD transgenic mouse brain tissue sections (Courtesy of Dr. Keqiang Ye, Emory University, U.S.A.). Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected APP N-terminal fragment 373N in brain tissue lysates from 5XFAD familial AD transgenic mice, but not from AEP-knockout 5XFAD mice (Courtesy of Dr. Keqiang Ye, Emory University, U.S.A.). Western Blotting Analysis: A representative lot detected an increased APP N-terminal fragment 373N in AEP-containing brain fractions from 5-month-old 5XFAD familial AD transgenic mice than wild-type mice (Zhang, Z., et al. (2015). Nat. Commun. 6:8762). Western Blotting Analysis: A representative lot detected an age-dependent increase brain APP N-terminal fragment 373N in mice. Upregulated APP 373N level was seen in human AD brain samples when compared with age-matched non-AD brains (Zhang, Z., et al. (2015). Nat. Commun. 6:8762). Western Blotting Analysis: A representative lot detected AEP-cleaved recombinant GST-APP N-terminal fragment, but not the uncleaved GST-APP or the cleaved C-terminal fragment (Zhang, Z., et al. (2015). Nat. Commun. 6:8762). Western Blotting Analysis: A representative lot detected AEP-cleaved APP N-terminal fragment 373N in untransfected, but not AEP siRNA-transfected, HEK293 cells. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the C-terminal end sequence of AEP-cleaved APP 373N fragment. |
| Epitope | extracellular domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antiserum detects the N-terminal fragment of AEP-cleaved APP at residue 373 (373N), but not the C-terminal fragment (373C) or uncleaved APP. The 373 numbering is based on isoforms L-APP677 and APP695/PreA4 695 (UniProt P05067-3 and P05067-4). Equivalent site is also present in isoforms APP770 (Asn448; P05067-1), L-APP696 (Asn392; P05067-5), APP714 (Asn392; P05067-6), L-APP733 (Asn429; P05067-7), APP751/PreA4 751 (Asn429; P05067-8), L-APP752 (Asn448; P05067-9), APP639 (Asn317; P05067-10), and isoform 11 (Asn424; P05067-11). Cleavage site is not present in APP305 (P05067-2). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Canine, Guinea Pig, Non-Human Primate, Porcine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Unpurified. |
| UniProt Number |
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| Molecular Weight | ~52/50 kDa observed. 48.53/48.53/48.53/42.35/42.35/46.40/46.40/48.53/34.14/47.87 (cleaved APP770/L-APP677/APP695/L-APP696/APP714/L-APP733/APP751/L-APP752/APP639/isoform 11 with signal peptide a.a. 1-17 removed) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in GST-APP-373 transfected cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antiserum detected APP(1-585), but not APP(1-373) or full-length APP, GST fusion exogenously expressed in HEK293 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |