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Millipore Anti-Pro-Trh, Cat. No. Abn1658, Is A Highly Specific Rabbit Polyclonal Antibody, That Targets Pro- Thyrotropin-Releasing Hormone And Has Been Tested In Immunofluorescence And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Pro-thyrotropin-releasing hormone (UniProt: P01150; also known as Pro-TRH, Prothyroliberin, TRH, TSH-releasing factor, Thyrotropin-releasing factor, TRF) is encoded by the Trh gene (Gene ID: 25569) in rat. TRH is a secreted hypothalamic protein that travels across the median eminence to the pituitary via the hypophyseal portal system. It functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. Pro-TRH (TRH-potentiating peptide: Ps4) is co-synthesized with TRH serves as a marker for TRH-containing neurons. Like TRH itself, pro-TRH is biologically active.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.09% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-pro-TRH, Cat. No. ABN1658, is a highly specific rabbit polyclonal antibody, that targets pro- Thyrotropin-releasing hormone and has been tested in Immunofluorescence and Western Blotting.
Key Applications
Immunofluorescence
Western Blotting
Application Notes
Immunofluorescence Analysis: A representative lot detected pro-TRH in median eminence of rat and L2 spinal cord tissues. (Courtesy of Dr. Martin Wessendorf at University of Minnesota).
Immunofluorescence Analysis: A representative lot detected pro-TRH in rat brain stem & rat spinal cord tissues. (Wessendorf, M.W., et. al. (1990). J Histochem Cyotchem. 38(12):1859-77).
Biological Information
Immunogen
A linear peptide corresponding to 10 amino acids
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This polyclonal antibody detects Thyrotropin-releasing hormone-containing neurons in Rat Hypothalamus and spinal cord.
Species Reactivity
Rat
Species Reactivity Note
Rat. Predicted to react with mouse due to sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_037178
Gene Symbol
Trh
Purification Method
Unpurified
UniProt Number
P01150
Molecular Weight
~29 kDa observed; 29.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in rat hypothalamus tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected pro-TRH in 10 µg of rat hypothalamus tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
