Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IHC, IP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1661 |
| Description | Anti-Membralin |
| Alternate Names |
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| Background Information | Membralin (UniProt: Q8CIV2; also known as Transmembrane protein 259) is encoded by the Tmem259 (also known as ORF61) gene (Gene ID: 216157) in murine species. Membralin is a highly conserved protein with several transmembrane regions. However, the sequence of membralin does not show any closely related genes. It has multiple glycosylation sites and cytosolic regions at both the N-terminal ends and C-terminal ends. It is expressed in multiple tissues, including brain and spinal cord. However, it is not found in heart, skeletal muscle, and spleen. In humans its membralin expression is reported in certain cancers, including the ovarian cancers. Membralin is reported to have a role in the ER-associated protein degradation (ERAD) pathway and is required for clearance of misfolded proteins in the endoplasmic reticulum (ER). It is also required for survival of motor neurons. Membralin mutant mice display increased basal ER stress and severe and early-onset motor neuron disease and die by postnatal day 5 or 6. (Ref.: Chen YC et al. (2005). Biochimica Biophysica Acta 1730(2): 96-102.) |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Membralin Antibody, Cat. No. ABN1661, is a highly specific rabbit polyclonal antibody that targets Membralin and has been tested in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhangs Lab at Sanford Burnham Prebys Medical Discovery Institute). Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Membralin in mouse neural progenitor cells. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhangs Lab at Sanford Burnham Prebys Medical Discovery Institute). Immunohistochemistry Analysis: 5.0 µg/mL from a representative lot detected Membralin in mouse spinal cord tissues. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhangs Lab at Sanford Burnham Prebys Medical Discovery Institute). Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhangs Lab at Sanford Burnham Prebys Medical Discovery Institute). |
| Biological Information | |
|---|---|
| Immunogen | A Myc-conjugated linear peptide from the N-terminal region of mouse membralin. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody specifically detects membralin in HEK293 cells overexpressing membralin. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A + Protein G |
| UniProt Number |
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| Molecular Weight | ~68 kDa observed; 63.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cells overexpressing membralin. Western Blotting Analysis: 1 µg/mL of this antibody detected Membralin in 4 µL of lysate from HEK293 cells transfected with mouse-myc-membralin and overexpressing Membralin. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |