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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1703 |
| Description | Anti-Tau, AEP-cleaved (N368) Antibody |
| Alternate Names |
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| Background Information | Microtubule-associated protein Tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. Neurofibrillary tangles (NFTs), composed of truncated and hyperphosphorylated Tau, are a common feature of aging-related neurodegenerative diseases, including Alzheimers disease (AD). In addition to caspases-mediated cleavage at Asp421, Tau can also be cleaved at Asn255 and Asn368 by the lysosomal cysteine proteinase asparagine endopeptidase (AEP). AEP is upregulated in aged brain, as well as n human AD brain and tau P301S–transgenic mice with synaptic pathology and behavioral impairments. AEP-mediated tau cleavage abolishes tau microtubule assembly function, induces tau aggregation and triggers neurodegeneration. AEP knockout or functional blockage in tau P301S mice leads to a reduction of tau hyperphosphorylation, protection against memory loss, and a reduction in tau P301S–triggered pathological and behavioral defects. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tau, AEP-cleaved (N368) Antibody is validated for use in Western Blotting for the detection of AEP-cleaved Tau N-terminal fragment (N368). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminal end sequence of AEP-cleaved human Tau N-terminal fragment N368 |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Specifically recognizes AEP-generated Tau fragments with N368 as the c-termus. Does not react with full-length Tau or Tau fragments with other cleaved c-termus. Uniprot P10636 lists 9 human tau isoforms produced by alternative splicing. The N368 designation was originally assigned to this polyclonal antiserum based on Asp368 of isoform 8 (Tau-F, Tau-4), which is equivalent to N685 of isoform 1 (PNS-tau), N279 of isoform 2 (Fetal-tau), N243 of isoform 3 (Tau-A), N308 of isoform 4 (Tau-B), N337 of isoform 5 (Tau-C, Tau-3), N310 of isoform 6 (Tau-D), N339 of isoform 7 (Tau-E), and N703 of isoform 9 (Tau-g). |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Bovine, Canine, Monkey, Mouse, Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed. Variable depending on the isoform and size of the target fragments. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting using purified Tau. Western Blotting Analysis: A 1:100,000 dilution of this antiserum specifically detected 1 µg of purified aa 1-368 Tau fragment, but not the full-length Tau protein. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |