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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN242 |
| Replaces | AB9886 |
| Description | Anti-Pannexin-1/PANX1 Antibody |
| Alternate Names |
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| Background Information | Pannexin-1 (UniProt Q96RD7; also known as Innexin) is encoded by the PANX1 (also known as MRS1, PX1) gene (ORF UNQ2529/PRO6028; Gene ID 24145) in human. Pannexin 1 is a high-conductance, voltage-gated channel protein widely expressed in many organs and tissues, including the sensory systems (eye, inner ear, taste buds, and the olfactory epithelium) and both neuronal and non-neuronal cell types. Three pannexin family genes (PANX1-3) have been identified in human and mouse genomes. Pannexin proteins share structural and functional similarities and play important roles in many physiological and pathological processes, such as ATP release, Ca2+ homeostasis, synaptic neurotransmitters release, apoptosis, and immunological response. Pannexin-1 deficiency causes caspase-3 activation and apoptosis induction of hair cells in cochlear spiral limbus (SLM), resulting in hearing loss among Panx1-/- mice. Panx1 is also expressed in the retina, lens and cornea of the eye, where it plays an important role in mediating the crosstalk between immune and neuronal cells. Pannexin-1 channel opening, frequently together with the activation of purinergic receptors by extracellular ATP, is reported to have a great implication in diverse pathological conditions, including ischemia, trauma, HIV infection, inflammation, migraine, and cancer cell growth. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Pannexin-1/PANX1 Antibody is an antibody against Pannexin-1/PANX1 for use in Western Blotting, Immunohistochemistry. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pannexin-1/PANX1 in human testis and mouse kidney tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human Pannexin-1/PANX1 C-terminal fragment. |
| Epitope | C-terminal |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with both spliced isoforms reported by UniProt (Q96RD7-1 & Q96RD7-2). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Chimpanzee, Gorilla based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~55 kDa observed. 55-58 kDa (membrane glycosylated) and 43 kDa (cytosolic non-glycosylated) expected. 48.1 kDa (isoform 1) & 47.6 kDa (isoform 2) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human cortex tissue lysate. Western Blotting Analysis: 0.1 µg/mL of this antibody detected Pannexin-1/PANX1 in 10 µg of human cortex tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |