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Millipore Anti-Olig-2 Antibody Is An Antibody Against Olig-2 For Use In Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Oligodendrocyte transcription factor 2 (UniProt Q9EQW6; also known as bHLHe19, Olg-2, Oligo2, RACK17, RK17) is encoded by the Olig2 (also known as Bhlhb1) gene (Gene ID 50913) in murine species. Olig2 is a basic helix-loop-helix (bHLH) transcription factor expressed in a spinal cord ventricular zone domain where it mediates the sequential generation of motoneurons and oligodendrocytes during embryogenesis. Olig2 also functions as an anti-neurogenic factor in pMN progenitors at early time points to sustain the cycling progenitor pool. The anti-neurogenicity of Olig2 is also responsible for its role in tumorigenesis, especially in brain tumors and leukemia. Due to its expression in glioblastoma and other diffuse gliomas, Olig2 is a useful diagnostic marker of these brain tumors.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in PBS and 0.1 % sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Olig-2 Antibody is an antibody against Olig-2 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry (Paraffin).
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry (Paraffin)
Application Notes
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Olig-2 in 500 µg of mouse brain tissue lysate. Immunohistochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in rat hindbrain tissue sections. Immunocytochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in PC12 cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to a sequence in the N-terminal region of mouse Olig-2.
Epitope
N-terminal region.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_058663
Gene Symbol
Olig2
Bhlhb1
Purification Method
Affinity Purfied
UniProt Number
Q9EQW6
Molecular Weight
~37 kDa observed. 32.40 kDa calculated.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Olig-2 in 10 µg of mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
