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Millipore Anti-Phlpp2 Antibody Is An Antibody Against Phlpp2 For Use In Western Blotting And Immunocytochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
PH domain leucine-rich repeat-containing protein phosphatase 2
PH domain leucine-rich repeat-containing protein phosphatase-like
PHLPP-like
Background Information
PH Domain And Leucine Rich Repeat Protein Phosphatase 2 is encoded by the PHLPP2 gene. It is known to catalyze the dephosphorylation of pSer473 of AKT1 and pSer660 of PRKCbeta-II, as well as pSer657 of PRKCalpha.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-PHLPP2 Antibody is an antibody against PHLPP2 for use in Western Blotting and Immunocytochemistry.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: 2.0 ug/mL from a representative lot detected PHLPP2 in HUVEC cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding within the PP2C-like domain of human PHLPP2.
Epitope
Within PP2C-like Domain
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Bovine based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_055835
Gene Symbol
PHLPP2
KIAA0931
PHLPPL
Purification Method
Affinity Purfied
UniProt Number
Q6ZVD8
Molecular Weight
~150 kDa observed. Predicted to cross-react to all isoforms. Isoform 2 (~107kDa) can possibly be detected at longer exposures/higher concentrations or in certain tissues.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected PHLPP2 in 10 µg of HUVEC cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
