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Millipore Detect Ube4b Using This Rabbit Polyclonal Anti-Ube4b Antibody, Cat. No. Abs1079, Validated For Use In Western Blotting And Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
Ubiquitin conjugation factor E4 B (UniProt O95155; also known as Homologous to yeast UFD2, Homozygously deleted in neuroblastoma 1, Ubiquitin fusion degradation protein 2) is encoded by the UBE4B (also known as E4, HDNB1, UBOX3, UFD2, UFD2A) gene (Gene ID 10277) in human. The turnover of plasma membrane proteins involves internalization and sorting by the endocytic pathway. Ubiquitin modification allows proteins to be recognized by endosomally associated protein complexes, enabling sorting of proteins to be degraded from those to be recycled. The ubiquitin ligase UBE4B is reported to interact with "hepatocyte growth factor-regulated tyrosine kinase substrate" (Hrs) of the "endosomal sorting complex required for transport 0" (ESCRT-0) complex at the endosomal membrane, where UBE4B ubiquitinates internalized EGFR to allow its recognition by ESCRT-0. The deubiquinating enzyme USP8 then associates with ESCRT-0 via binding to "signal-transducing adaptor molecule" (STAM), while UBE4B dissociates from ESCRT-0. ESCRT-I and II then bind to cargo and facilitate membrane budding inward toward the lumen, whereas ESCRT-III mediates the dissociation of ESCRTs from endosomal membranes and membrane scission.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing Tris-HCl with 0.05% sodium azide with 50% glycerol.
Quality Level
MQ100
Applications
Application
Detect UBE4B using this rabbit Polyclonal Anti-UBE4B Antibody, Cat. No. ABS1079, validated for use in Western Blotting and ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Western Blotting Analysis: A representative lot detected recombinant UBE4B, endogenous UBE4B in STAM immunoprecipitate from HeLa cells, and siRNA-mediated UBE4B depletion in HeLa cells (Sirisaengtaksin, N., et al. (2014). J Biol Chem. 289(5):3026-3039). Immunocytochemistry Analysis: A representative lot detected UBE4B cellular localization in HeLa cells with or without EGF-EGFR internalization (Sirisaengtaksin, N., et al. (2014). J Biol Chem. 289(5):3026-3039).
Biological Information
Immunogen
Recombinant protein corresponding to human UBE4B.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This antibody is expected to react with all 4 spliced isoforms of human UBE4B protein.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001099032
Gene Symbol
UBE4B
HDNB1
KIAA0684
UFD2
Purification Method
Affinity Purfied
UniProt Number
O95155
Molecular Weight
~130 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected UBE4B in 10 µg of K562 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
